? Card: Activates the item without consuming it, allowing for potential multiple uses.
Book of Virtues: Spawns a random wisp. For the rest of the run, a random wisp spawns after clearing a room if the one given by the item was destroyed, including the replacement wisps given by this effect.
Diplopia: Spawns two Eden's Souls that will both drop 2 items when used.
Wild Card: Gives the effect of Eden's Soul if it was the last item/card used.
Interactions
The Battery: Eden's Soul cannot be activated twice with a double charge.
Car Battery: Does not affect Eden's Soul. Only two items will spawn upon use.
Void: If absorbed, Eden's Soul is stored, and allows for a single use in a desired location. Also effectively reduces charge time to 6 rooms.
Car Battery + Void: Eden's Soul is Voided and then immediately consumed, spawning 2 items from the current item pool. This is likely to be the Angel Room as that is where Eden’s Soul is usually found. Effectively removes the charge time of Eden’s Soul.
If Car Battery is picked up after Eden's Soul was absorbed, the next Void use will spawn 2 item pedestals and immediately Void them.
For the soul stone with a similar name, see Soul of Eden.
Unlike 
Mega Blast, this item can be charged with a single 
Lil' Battery.
? Card: Activates the item without consuming it, allowing for potential multiple uses.
Book of Virtues: Spawns a random wisp. For the rest of the run, a random wisp spawns after clearing a room if the one given by the item was destroyed, including the replacement wisps given by this effect.
Diplopia: Spawns two Eden's Souls that will both drop 2 items when used.
Wild Card: Gives the effect of Eden's Soul if it was the last item/card used.
The Battery: Eden's Soul cannot be activated twice with a double charge.
Car Battery: Does not affect Eden's Soul. Only two items will spawn upon use.
Void: If absorbed, Eden's Soul is stored, and allows for a single use in a desired location. Also effectively reduces charge time to 6 rooms.
Alabaster Box and 
Broken Glass Cannon, that:
Eden Unlocks 
Delirium Unlocks 
The Binding of Isaac: Rebirth
