Soy Milk: Tears now travel in a double helix pattern.
Tractor Beam: The altered pattern tears travel in is confined to the beam allowing Isaac to use its benefits without losing accuracy.
Golden Trinket / Mom's Box / Wiggle Worm: In addition to further stat increases, each effect/trinket copy causes the path of tears to become even wigglier.
Tech X: Rings take a star-shape, slightly increasing their area of effect.
Wiggle Worm was a passive item in the original Binding of Isaac. The likely reason for the change to a trinket is the situational usefulness of this shot pattern, along with the new ability to drop trinkets on demand. It took ItemID #59, which is now unused.