Chapter 1 (Bosses)

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Normal mode only Normal / Hard mode only Hard Bosses

BasementBasement

Boss Dingle portrait.png
Boss The Duke of Flies portrait.png
 Boss Gemini portrait.png
Boss Steven portrait.png
Dingle The Duke of Flies Gemini Steven Needs to be unlocked
Boss Larry Jr. portrait.png
 Boss Monstro portrait.png
Boss Gurglings portrait.png
 Boss Famine portrait.png
Larry Jr. Monstro Gurglings Famine Needs to be unlocked
Boss Headless Horseman portrait.png
Boss The Fallen portrait.png
Boss Dangle portrait.png
Boss Little Horn portrait.png
Headless Horseman Needs to be unlocked The Fallen Devil Room Icon.png Dangle (except in Rebirth) Needs to be unlocked Little Horn (except in Rebirth) Needs to be unlocked
Boss Turdlings portrait.png
Boss Rag Man portrait.png
Boss Baby Plum portrait.png
Turdlings (except in Rebirth) Needs to be unlocked Rag Man (in Afterbirth and Afterbirth †) Needs to be unlocked Baby Plum (in Repentance and Repentance+) Needs to be unlocked

CellarCellar

Boss Blighted Ovum portrait.png
Boss The Duke of Flies portrait.png
Boss The Haunt portrait.png
Boss Pin portrait.png
Blighted Ovum The Duke of Flies The Haunt Pin
Boss Widow portrait.png
Boss Fistula portrait.png
Boss Famine portrait.png
Boss Headless Horseman portrait.png
Widow Fistula (except in Repentance and Repentance+) Famine Needs to be unlocked Headless Horseman Needs to be unlocked
Boss The Fallen portrait.png
Boss Little Horn portrait.png
Boss Rag Man portrait.png
Boss Baby Plum portrait.png
The Fallen Devil Room Icon.png Little Horn (except in Rebirth) Needs to be unlocked Rag Man (except in Rebirth) Needs to be unlocked Baby Plum (in Repentance and Repentance+) Needs to be unlocked

(except in Rebirth)Burning BasementBurning Basement

Boss The Duke of Flies portrait.png
Boss Larry Jr. portrait.png
Boss Monstro portrait.png
 Boss The Haunt portrait.png
The Duke of Flies Larry Jr. Monstro The Haunt (except in Repentance and Repentance+)
Boss Famine portrait.png
Boss Headless Horseman portrait.png
Boss The Fallen portrait.png
Boss Little Horn portrait.png
Famine Needs to be unlocked Headless Horseman Needs to be unlocked The Fallen Devil Room Icon.png Little Horn Needs to be unlocked
Boss Rag Man portrait.png
Boss Dingle portrait.png
Boss Gurglings portrait.png
Boss Gemini portrait.png
Rag Man Needs to be unlocked Dingle (in Repentance and Repentance+) Gurglings (in Repentance and Repentance+) Gemini (in Repentance and Repentance+)
Boss Steven portrait.png
Boss Dangle portrait.png
Boss Turdlings portrait.png
Boss Baby Plum portrait.png
Steven (in Repentance and Repentance+) Needs to be unlocked Dangle (in Repentance and Repentance+) Needs to be unlocked Turdlings (in Repentance and Repentance+) Needs to be unlocked Baby Plum (in Repentance and Repentance+) Needs to be unlocked

Notes

Boss Monstro portrait.png Bosses Boss Monstro portrait.png
Chapter 1
BasementCellarBurning Basement 		Boss Monstro ingame.pngMonstro • Boss Gemini ingame.pngGemini • Boss Steven ingame.pngSteven • Boss Dingle ingame.pngDingle • Gurgling.pngGurglings • Boss Larry Jr. ingame.pngLarry Jr. • Boss The Duke of Flies ingame.pngThe Duke of Flies • Boss Widow ingame.pngWidow • Boss Blighted Ovum ingame.pngBlighted Ovum • Boss The Haunt ingame.pngThe Haunt • Boss Pin ingame.pngPin • Boss Famine ingame.pngFamine • Boss The Fallen ingame.pngThe Fallen • Boss Headless Horseman ingame.pngHeadless Horseman
(except in Rebirth) Boss Dangle ingame.pngDangle • Boss Turdlings ingame.pngTurdlings • Boss Little Horn ingame.pngLittle Horn • Boss Rag Man ingame.pngRag Man
(in Repentance and Repentance+)Boss Baby Plum ingame.pngBaby Plum
(in Repentance and Repentance+) Chapter 1.5
DownpourDross 		Boss The Rainmaker ingame.pngThe Rainmaker • Boss Min-Min ingame.pngMin-Min • Boss Lil Blub (Downpour) ingame.pngLil Blub • Boss Wormwood (Downpour) ingame.pngWormwood • Boss Clog ingame.pngClog • Boss Colostomia ingame.pngColostomia • Boss Turdlet ingame.pngTurdlet
Chapter 2
CavesCatacombsFlooded Caves 		Boss Fistula ingame.pngFistula • Boss Chub ingame.pngChub • Boss C.H.A.D. ingame.pngC.H.A.D. • Boss Gurdy ingame.pngGurdy • Boss Mega Fatty ingame.pngMega Fatty • Boss Mega Maw ingame.pngMega Maw • Boss Gurdy Jr. ingame.pngGurdy Jr. • Boss Peep ingame.pngPeep • Boss The Husk ingame.pngThe Husk • Boss The Hollow ingame.pngThe Hollow • Boss Carrion Queen ingame.pngCarrion Queen • Boss Dark One ingame.pngDark One • Boss Polycephalus ingame.pngPolycephalus • Boss The Wretched ingame.pngThe Wretched • Boss Pestilence ingame.pngPestilence • Boss The Fallen ingame.pngThe Fallen • Boss Headless Horseman ingame.pngHeadless Horseman
(except in Rebirth) Boss The Stain ingame.pngThe Stain • Boss The Frail ingame.pngThe Frail • Boss The Forsaken ingame.pngThe Forsaken
(except in Rebirth and Afterbirth) Boss Big Horn ingame.pngBig Horn • Boss Rag Mega ingame.pngRag Mega
(in Repentance and Repentance+)Boss Bumbino ingame.pngBumbino
(in Repentance and Repentance+) Chapter 2.5
MinesAshpit 		Boss Reap Creep ingame.pngReap Creep • Boss Tuff Twins ingame.pngTuff Twins • Boss Hornfel ingame.pngHornfel • Boss Great Gideon ingame.pngGreat Gideon • Boss The Pile ingame.pngThe Pile • Boss The Shell ingame.pngThe Shell • Boss Singe ingame.pngSinge • Boss Clutch ingame.pngClutch
Chapter 3
DepthsNecropolisDank Depths 		Boss Monstro II ingame.pngMonstro II • Boss Gish ingame.pngGish • Boss The Cage ingame.pngThe Cage • Boss The Gate ingame.pngThe Gate • Boss Loki ingame.pngLoki • Boss The Adversary ingame.pngThe Adversary • Boss The Bloat ingame.pngThe Bloat • Boss Mask of Infamy ingame.pngMask of Infamy • Boss War ingame.pngWar • Boss The Fallen ingame.pngThe Fallen • Boss Headless Horseman ingame.pngHeadless Horseman • Boss Mom ingame.pngMom
(except in Rebirth) Boss Brownie ingame.pngBrownie
(except in Rebirth and Afterbirth) Boss Sisters Vis ingame.pngSisters Vis
(in Repentance and Repentance+) Boss Reap Creep ingame.pngReap Creep • Boss The Pile ingame.pngThe Pile
(in Repentance and Repentance+) Chapter 3.5
MausoleumGehenna 		Boss The Siren ingame.pngThe Siren • Boss The Heretic ingame.pngThe Heretic • Boss The Visage ingame.pngThe Visage • Boss The Horny Boys ingame.pngThe Horny Boys • Boss Mom ingame.pngMom • Boss Mom's Heart ingame.pngMom's Heart
Chapter 4
WombUteroScarred Womb 		Boss Blastocyst ingame.pngBlastocyst • Boss Mama Gurdy ingame.pngMama Gurdy • Boss Scolex ingame.pngScolex • Boss Mr. Fred ingame.pngMr. Fred • Boss Lokii ingame.pngLokii • Boss The Bloat ingame.pngThe Bloat • Boss Daddy Long Legs ingame.pngDaddy Long Legs • Boss Triachnid ingame.pngTriachnid • Boss Teratoma ingame.pngTeratoma • Boss Death ingame.pngDeath • Boss Conquest ingame.pngConquest • Boss The Fallen ingame.pngThe Fallen • Boss Headless Horseman ingame.pngHeadless Horseman • Boss Mom's Heart ingame.pngMom's Heart • Boss It Lives! ingame.pngIt Lives!
(except in Rebirth and Afterbirth)Boss The Matriarch ingame.pngThe Matriarch
(except in Rebirth) ???
??? 		Boss Hush ingame.pngHush
(in Repentance and Repentance+) Corpse
Corpse 		Boss Chimera ingame.pngChimera • Boss The Scourge ingame.pngThe Scourge • Boss Rotgut ingame.pngRotgut • Boss Mother secondphase ingame.pngMother
Chapter 5
SheolCathedral 		Boss Satan ingame.pngSatan • Boss Isaac ingame.pngIsaac
Chapter 6
Dark RoomChest 		Boss The Lamb ingame.pngThe Lamb • Boss ??? ingame.png??? • Mega Satan Head ingame.pngMega Satan
(except in Rebirth and Afterbirth) The Void
The Void 		Boss Delirium ingame.pngDelirium
(in Repentance and Repentance+) Home
Home 		Boss Dogma ingame.pngDogma • Boss Ultra Famine ingame.pngUltra Famine • Boss Ultra Pestilence ingame.pngUltra Pestilence • Boss Ultra War ingame.pngUltra War • Boss Ultra Death ingame.pngUltra Death • Boss The Beast ingame.pngThe Beast
(except in Rebirth) Greed Mode
Ultra Greed 		Boss Ultra Greed ingame.pngUltra Greed
(except in Rebirth and Afterbirth)Boss Ultra Greedier ingame.pngUltra Greedier
SmallIsaac.pngThe Binding of Isaac: RebirthSmallIsaac.png
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