1up!
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1up! is a passive item.
Effects
- Gives Isaac an extra life. Upon death, he will respawn in the previous room with full health, after which 1up! will disappear.
- If Isaac has no Red Heart Containers, he'll respawn with a
Half Soul Heart instead.
- If Isaac has no Red Heart Containers, he'll respawn with a
- The 1up! mushroom begins following Isaac like a familiar upon pickup, but doesn't do anything. It disappears when its life is used up.
This item belongs to the 
Fun Guy set. Collecting three items from this set will transform Isaac into a walking mushroom.
Notes
1up! can spawn from destroying 
Revive Machines.- Resurrection items activate in a set order. 1up! is
1st 
2nd in this order after 
Soul of Lazarus. -
??? / 
Tainted ??? / 
Tainted Bethany / 
Tainted Forgotten will each respawn with 3 
Soul Hearts. 
The Forgotten will respawn with 3 / 2 filled 
Bone Hearts and 
The Soul with whatever Soul and Black Hearts he had on death.
- Dying as The Soul respawns him with a Half Soul Heart / one Soul Heart and The Forgotten with whatever Bone Hearts he had on death.
- Dying as The Soul respawns him with
-
Black Judas / 
Tainted Judas will respawn with 2 
Black Hearts. -
Tainted Jacob will respawn in his ghost form if he had been turned into his ghost form before dying. - Familiars that follow behind Isaac have a certain priority in how close their position is to him over other familiars, with 1up! having the lowest priority at
6th / 8th and thus being farther from Isaac than familiars of different priorities.
In-game Footage
Trivia
- This is a reference to the Super Mario franchise, wherein collecting a green mushroom would give the player an extra life.
- 1up!, along with
Magic Mushroom, 
Mini Mush, 
Super Bandage, and 
Mega Mush is one of the few items that make a unique sound upon pickup.
 Mushrooms
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| Items |
| Trinkets |
 Revival Items
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| Items |
| Trinkets |
| Soul stones |
 The Binding of Isaac: Rebirth
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